Visual Detection of Bacterial Pathogens via PNA-Based Padlock Probe Assembly and Isothermal Amplification of DNAzymes
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چکیده
منابع مشابه
Visual Detection of Bacterial Pathogens via PNA-Based Padlock Probe Assembly and Isothermal Amplification of DNAzymes
We have developed a self-reporting isothermal system for visual bacterial pathogen detection with single base resolution. The new DNA diagnostic is based on combination of peptide nucleic acid (PNA) technology, rolling circle amplification (RCA) and DNAzymes. PNAs are used as exceedingly selective chemical tools that bind genomic DNA at a predetermined sequence under nondenaturing conditions. A...
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Department of Electrical and Computer Urbana-Champaign, Urbana, IL 61801, USA Micro and Nanotechnology Laboratory, Un Urbana, IL 61801, USA Design and Technology Platform, Taiwan Hsinchu, Taiwan Research and Ecosystem, Delta Electronics Department of Bioengineering, University o IL 61802, USA. E-mail: [email protected] † Electronic supplementary informa 10.1039/c6ra19685c Cite this: RSC Adv....
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2014
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac5018748